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smartscribetm reverse transcriptase  (TaKaRa)


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    TaKaRa smartscribetm reverse transcriptase
    Smartscribetm Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/smartscribetm+reverse+transcriptase/SMARTScribe+Reverse+Transcriptase/med_rxiv__64898__2026__05__02__26352058-240-43-46
    Average 96 stars, based on 1128 article reviews
    smartscribetm reverse transcriptase - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Induction of multiple HIV-1 neutralizing B Cell Precursors in Humans
    Article Snippet: Briefly, RNA was combined with dNTPs (NEB, Cat. #N0447L), a template-switching oligonucleotide (TSO; containing 5’ padding and a plate-level barcode), and constant-region reverse primers specific for IgG, Igκ, and Igλ. .. Reactions were denatured at 72°C for 3 min, chilled on ice for 2 min, and supplemented with an RT mix containing 5x RT buffer (final 1x), 20 mM DTT (final 2 mM), RNaseOUTTM RNase Inhibitor (Thermo Fisher Scientific, Cat. #10777-019; final 1x), and SMARTScribeTM Reverse Transcriptase (Takara, Cat. #639538). ..

    Article Title: Integrated m6A methylome and transcriptome profiling of mRNAs and lncRNAs in nasal mucosal epithelial cells of allergic rhinitis patients undergoing allergen-specific immunotherapy.
    Article Snippet: .. The immunoprecipitated RNA was reverse-transcribed into first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat. 634414, Tokyo, Japan), followed by adapter ligation and second-strand cDNA synthesis via polymerase chain reaction (PCR) under the following conditions: initial denaturation at 94°C for 1 minute; 5 cycles of 98°C for 15 seconds (denaturation), 55°C for 15 seconds (annealing), and 68°C for 30 seconds (extension); and a final extension at 68°C for 2 minutes. ..

    Article Title: Functional Convergence of Genetically Diverse B-Cell Receptors in Simian-HIV Infected Rhesus Macaques
    Article Snippet: Briefly, RNA samples were mixed with dNTPs (NEB, Cat# N0447L), a template-switching oligo (TSO, with 5’ padding and one of the unique plate-barcoding sequences), and reverse primers specific to the constant regions of IgG, Igκ, Igλ, and optionally IgM (see Table S7 for primer sequences, with “Purpose” column as “BCR V(D)J sequencing”). .. The mixture was denatured at 72°C for 3 minutes (min) and annealed on ice for 2 min. A reverse transcription (RT) pre-mixture was then added, comprising 1/5 final volume of 5× RT buffer, 1/10 final volume of 20 mM DTT, 1/20 final volume of RNaseOUTTM RNase Inhibitor (ThermoFisher, Cat# 10777-019), and 1/20 final volume of SMARTScribeTM Reverse Transcriptase (TaKaRa Bio, Cat# 639538). ..

    Article Title: Histone lactylation maintains bovine early embryo development via regulating embryonic genome activation.
    Article Snippet: Total RNA quantity and purity were analyzed with a Thermo Fisher Qubit 3.0 (Thermo Fisher Scientific, Waltham, MA, USA). polyA RNA in the samples was used as the template, and the oligo(dT) sequence with an adapter was used as the primer. .. Single-strand synthesis was carried out using SMARTScribeTM reverse transcriptase (TaKaRa, Kyoto, Japan). ..

    Article Title: Profiles and roles of N6-methyladenosine modification in bovine oocyte maturation.
    Article Snippet: .. The immunoprecipitated (IP) RNA was reverse-transcribed to generate first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat.634414, Japan), followed by adapter ligation and second-stranded cDNA synthesis with PCR under the following conditions: 94 °C for 1 min; 5 cycles of 98 °C for 15 s, 55 °C for 15 s, and 68 °C for 30 s; and a final extension at 68 °C for 2 min. ..

    Article Title: The discovery of missing taxane C13 α - O -deacetylases re-delineates the biosynthetic pathway of paclitaxel
    Article Snippet: .. RNA isolated from T. × media cell cultures was converted into cDNA using the SMARTScribeTM Reverse Transcriptase (Takara). ..

    Article Title: Profiles and roles of N6-methyladenosine modification in bovine oocyte maturation
    Article Snippet: .. The immunoprecipitated (IP) RNA was reverse-transcribed to generate first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat.634414, Japan), followed by adapter ligation and second-stranded cDNA synthesis with PCR under the following conditions: 94 °C for 1 min; 5 cycles of 98 °C for 15 s, 55 °C for 15 s, and 68 °C for 30 s; and a final extension at 68 °C for 2 min. ..

    Article Title: Histone lactylation maintains bovine early embryo development via regulating embryonic genome activation
    Article Snippet: Total RNA quantity and purity were analyzed with a Thermo Fisher Qubit 3.0 (Thermo Fisher Scientific, Waltham, MA, USA). polyA RNA in the samples was used as the template, and the oligo(dT) sequence with an adapter was used as the primer. .. Single-strand synthesis was carried out using SMARTScribeTM reverse transcriptase (TaKaRa, Kyoto, Japan). ..

    Immunoprecipitation:

    Article Title: Integrated m6A methylome and transcriptome profiling of mRNAs and lncRNAs in nasal mucosal epithelial cells of allergic rhinitis patients undergoing allergen-specific immunotherapy.
    Article Snippet: .. The immunoprecipitated RNA was reverse-transcribed into first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat. 634414, Tokyo, Japan), followed by adapter ligation and second-strand cDNA synthesis via polymerase chain reaction (PCR) under the following conditions: initial denaturation at 94°C for 1 minute; 5 cycles of 98°C for 15 seconds (denaturation), 55°C for 15 seconds (annealing), and 68°C for 30 seconds (extension); and a final extension at 68°C for 2 minutes. ..

    Article Title: Profiles and roles of N6-methyladenosine modification in bovine oocyte maturation.
    Article Snippet: .. The immunoprecipitated (IP) RNA was reverse-transcribed to generate first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat.634414, Japan), followed by adapter ligation and second-stranded cDNA synthesis with PCR under the following conditions: 94 °C for 1 min; 5 cycles of 98 °C for 15 s, 55 °C for 15 s, and 68 °C for 30 s; and a final extension at 68 °C for 2 min. ..

    Article Title: Profiles and roles of N6-methyladenosine modification in bovine oocyte maturation
    Article Snippet: .. The immunoprecipitated (IP) RNA was reverse-transcribed to generate first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat.634414, Japan), followed by adapter ligation and second-stranded cDNA synthesis with PCR under the following conditions: 94 °C for 1 min; 5 cycles of 98 °C for 15 s, 55 °C for 15 s, and 68 °C for 30 s; and a final extension at 68 °C for 2 min. ..

    Adapter Ligation:

    Article Title: Integrated m6A methylome and transcriptome profiling of mRNAs and lncRNAs in nasal mucosal epithelial cells of allergic rhinitis patients undergoing allergen-specific immunotherapy.
    Article Snippet: .. The immunoprecipitated RNA was reverse-transcribed into first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat. 634414, Tokyo, Japan), followed by adapter ligation and second-strand cDNA synthesis via polymerase chain reaction (PCR) under the following conditions: initial denaturation at 94°C for 1 minute; 5 cycles of 98°C for 15 seconds (denaturation), 55°C for 15 seconds (annealing), and 68°C for 30 seconds (extension); and a final extension at 68°C for 2 minutes. ..

    Article Title: Profiles and roles of N6-methyladenosine modification in bovine oocyte maturation.
    Article Snippet: .. The immunoprecipitated (IP) RNA was reverse-transcribed to generate first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat.634414, Japan), followed by adapter ligation and second-stranded cDNA synthesis with PCR under the following conditions: 94 °C for 1 min; 5 cycles of 98 °C for 15 s, 55 °C for 15 s, and 68 °C for 30 s; and a final extension at 68 °C for 2 min. ..

    Article Title: Profiles and roles of N6-methyladenosine modification in bovine oocyte maturation
    Article Snippet: .. The immunoprecipitated (IP) RNA was reverse-transcribed to generate first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat.634414, Japan), followed by adapter ligation and second-stranded cDNA synthesis with PCR under the following conditions: 94 °C for 1 min; 5 cycles of 98 °C for 15 s, 55 °C for 15 s, and 68 °C for 30 s; and a final extension at 68 °C for 2 min. ..

    cDNA Synthesis:

    Article Title: Integrated m6A methylome and transcriptome profiling of mRNAs and lncRNAs in nasal mucosal epithelial cells of allergic rhinitis patients undergoing allergen-specific immunotherapy.
    Article Snippet: .. The immunoprecipitated RNA was reverse-transcribed into first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat. 634414, Tokyo, Japan), followed by adapter ligation and second-strand cDNA synthesis via polymerase chain reaction (PCR) under the following conditions: initial denaturation at 94°C for 1 minute; 5 cycles of 98°C for 15 seconds (denaturation), 55°C for 15 seconds (annealing), and 68°C for 30 seconds (extension); and a final extension at 68°C for 2 minutes. ..

    Article Title: Profiles and roles of N6-methyladenosine modification in bovine oocyte maturation.
    Article Snippet: .. The immunoprecipitated (IP) RNA was reverse-transcribed to generate first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat.634414, Japan), followed by adapter ligation and second-stranded cDNA synthesis with PCR under the following conditions: 94 °C for 1 min; 5 cycles of 98 °C for 15 s, 55 °C for 15 s, and 68 °C for 30 s; and a final extension at 68 °C for 2 min. ..

    Article Title: Profiles and roles of N6-methyladenosine modification in bovine oocyte maturation
    Article Snippet: .. The immunoprecipitated (IP) RNA was reverse-transcribed to generate first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat.634414, Japan), followed by adapter ligation and second-stranded cDNA synthesis with PCR under the following conditions: 94 °C for 1 min; 5 cycles of 98 °C for 15 s, 55 °C for 15 s, and 68 °C for 30 s; and a final extension at 68 °C for 2 min. ..

    Polymerase Chain Reaction:

    Article Title: Integrated m6A methylome and transcriptome profiling of mRNAs and lncRNAs in nasal mucosal epithelial cells of allergic rhinitis patients undergoing allergen-specific immunotherapy.
    Article Snippet: .. The immunoprecipitated RNA was reverse-transcribed into first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat. 634414, Tokyo, Japan), followed by adapter ligation and second-strand cDNA synthesis via polymerase chain reaction (PCR) under the following conditions: initial denaturation at 94°C for 1 minute; 5 cycles of 98°C for 15 seconds (denaturation), 55°C for 15 seconds (annealing), and 68°C for 30 seconds (extension); and a final extension at 68°C for 2 minutes. ..

    Article Title: Profiles and roles of N6-methyladenosine modification in bovine oocyte maturation.
    Article Snippet: .. The immunoprecipitated (IP) RNA was reverse-transcribed to generate first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat.634414, Japan), followed by adapter ligation and second-stranded cDNA synthesis with PCR under the following conditions: 94 °C for 1 min; 5 cycles of 98 °C for 15 s, 55 °C for 15 s, and 68 °C for 30 s; and a final extension at 68 °C for 2 min. ..

    Article Title: Profiles and roles of N6-methyladenosine modification in bovine oocyte maturation
    Article Snippet: .. The immunoprecipitated (IP) RNA was reverse-transcribed to generate first-strand cDNA using SMARTScribeTM Reverse Transcriptase (CloneTech, cat.634414, Japan), followed by adapter ligation and second-stranded cDNA synthesis with PCR under the following conditions: 94 °C for 1 min; 5 cycles of 98 °C for 15 s, 55 °C for 15 s, and 68 °C for 30 s; and a final extension at 68 °C for 2 min. ..

    Isolation:

    Article Title: The discovery of missing taxane C13 α - O -deacetylases re-delineates the biosynthetic pathway of paclitaxel
    Article Snippet: .. RNA isolated from T. × media cell cultures was converted into cDNA using the SMARTScribeTM Reverse Transcriptase (Takara). ..



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